【摘 要】
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Disease-related mRNAs have been accepted as specific biomarkers for disease diagnosis and gene therapy.Adenocarcinoma human alveolar basal epithelial cells come from cancerous lung tissue and have a h
【机 构】
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College of Chemistry,Sichuan University,Chengdu,610064,China
【出 处】
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2016年分析化学前沿国际研讨会及中美分析化学研讨会
论文部分内容阅读
Disease-related mRNAs have been accepted as specific biomarkers for disease diagnosis and gene therapy.Adenocarcinoma human alveolar basal epithelial cells come from cancerous lung tissue and have a high expression level of c-raf-1 mRNA.[1] Nanopore sensor shows a potential capability to probe nucleic acid hybridization and differentiate nanostructures of nucleic strand at the single-molecule level.[2,3] In this work,a DNA probe was designed to have a hairpin-loop structure whose loop part can specifically hybridize with the target mRNA.As shown in Fig 1,the linear DNA(poly(dA)36)translocations the α-hemolysin(α-HL)protein nanopore fast with a short dwell time of 0.4 ms.In contrast,the DNA probe,having a hairpin-loop structure,has a longer dwell time of 32.4 ms.Because of the specific hybridization between the DNA probe and the target mRNA,the dwell time turns to 201.2 ms.Therefore,the dwell time can be used to easily distinguish the target mRNA from other nucleic acids depending on the specific hybridization.The advantages of the protein nanopore such as label-free,quick detection and single-molecule level have made it an ideal approach for sensing mRNA,which would facilitate the development of disease diagnosis and gene therapy.
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