【摘 要】
：
Raf-1激酶抑制蛋白(Raf-1 kinase inhibitor protein,RKIP)在多个细胞信号转导通路中起着关键性的调控作用,并且与多种肿瘤密切相关,已经发展成肿瘤标志物.我们利用AUTODOCK
【机 构】
：
厦门大学化学化工学院化学生物学系生物核磁共振实验室,厦门361005厦门大学生命科学院,厦门361005
【出 处】
：
中国细胞生物学学会2013年全国学术大会
论文部分内容阅读
Raf-1激酶抑制蛋白(Raf-1 kinase inhibitor protein,RKIP)在多个细胞信号转导通路中起着关键性的调控作用,并且与多种肿瘤密切相关,已经发展成肿瘤标志物.我们利用AUTODOCK软件从现有的药物分子库里筛选出4种与RKIP有较强相互作用的药物,Suramin,Clofazimine,Glimepiride,Pranlukast.并利用荧光光谱和NMR技术研究了这些配体与RKIP的相互作用强度和结合位点.荧光实验研究表明,它们的解离常数Kd分别为:3.6 uM,2.32 uM,0.74 uM,5.17 uM.
其他文献
Stem cells in adult tissues interact with surrounding stromal cells (or niche) via signaling pathways to precisely balance stem cell self-renewal and differenti
Generation of hematopoietic stem and progenitor cells in vitro,especially generation of safe therapeutic hematopoietic stem cells,still remain inefficient becau
不同组织具有一定的特征弹性,细胞与其所在组织的弹性相适应;即:多种细胞的形态、分化或功能维持都受细胞外基质(Extracellular Matrix,ECM)弹性调控.细胞如何感知ECM的弹性?
The Drosophila ovarian germline stem cells (GSCs) constantly experience self-renewal and differentiation,ensuring the female fertility throughout life.The balan
Previous research has revealed that Wntl 0b activates canonical Wnt signaling,which is integral to melanocyte differentiation in hair follicles (HFs).However,th
HOX基因簇基因编码调控生物发育过程模式形成的重要转录因子,其在脊椎动物中呈现严格的时空共线性表达的特征.在胚胎干细胞中,HOX基因簇处于双价染色体(bivalent chromatin)
Jhdm1d位于小鼠6号染色体,编码含有JmjC结构域的组蛋白去甲基化酶I同系物D,对H3K9me2 (Histone H3 lysine 9 dimethylation)和H3K27me2 (Histone H3 lysine 27 dimethylation
Follicular helper T cells have recently been defined as a separate lineage of effeetor CD4 T cells that are specialized in providing help to B cells for the for
T cell is the major component of the adaptive immune system and its activation depends on the stimulation from exogenous antigen.We for the first time revealed
T细胞是在抗细菌/病毒感染、抗肿瘤、自身免疫、移植排斥等许多重要生理病理过程中起着关键作用的免疫细胞,T细胞自身的癌变也导致预后极差的白血病或淋巴瘤.因此,全面系统地