Background: Staphylococcus capitis is an emerging opportunistic pathogen of humans,and found as a colonizer of the human gut.Here,we report a case of S.capitis subsp.urealyticus infection.The strain H-02 was isolated from the blood culture of a patient with sigmoid colon cancer.It was found to be resistant to some important antibiotics,such as linezolid,a highly effective antimicrobial against clinically important Staphylococci pathogens.However,data on the genetic resistance mechanisms in S.capitis subsp.urealyticus are only sparsely available.Results: The draft genome of S.capitis subsp.urealyticus grain H-02 was sequenced by using next-generation sequencing technologies.Sequence data assembly revealed a genome size of 2,792,851 bp with a G + C content of 33.23%.Genome annotation revealed the presence of antibiotic resistance genes conferring resistance against some of the tested antibiotics as well as non-tested antibiotics.The genome also possesses a lot of genes that may be related to multidrug resistance.Whole genome comparison of the H-02 with five other S.capitis grains showed that some functional regions are highly homologous between the six assemblies made herein.Conclusion: The presence of a plethora of genes responsible for antibiotic resistance suggests that strain H-02 could present a potential threat to human health.The comparative genomic analysis of S.capitis strains presented in this study is important for better understanding of multidrug resistance in S.capitis.