德氏乳杆菌保加利亚亚种ND02活的非可培养态的诱导和复苏

来源 :第八届全国微生物资源学术暨国家微生物资源平台运行服务研讨会 | 被引量 : 0次 | 上传用户：yhl_2011

【摘要】

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【作者】

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王亚利王俊国张和平包秋华

【机构】

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内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018

【出处】

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第八届全国微生物资源学术暨国家微生物资源平台运行服务研讨会

【发表日期】

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2016年10期

【关键词】

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保加利亚乳杆菌ND02 VBNC态诱导复苏

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　　本文对一株具有优良发酵特性的德氏乳杆菌保加利亚亚种ND02(简写：保加利亚乳杆菌ND02)进行了活的非可培养态(Viable but nonculturable,VBNC)的诱导和复苏的初步研究.将活化的保加利亚乳杆菌ND02在不同复合条件下进行诱导,同时采用平板计数法和荧光显微镜两种计数方法对其进行检测,将诱导成功的样本通过改变温度及改变培养基成份进行复苏试验.结果表明：保加利亚乳杆菌ND02在一定条件下存在VBNC态,在液体MRS中4℃诱导190天全部进入VBNC态,并发现脱脂乳(10％+0.1％酵母粉)是使保加利亚乳杆菌ND02的VBNC态复苏为可培养态的最有效方法.这为保加利亚乳杆菌ND02在发酵剂制备过程中提高活性提供一定的参考依据.

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德氏乳杆菌保加利亚亚种ND02活的非可培养态的诱导和复苏

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