Advanced radiotherapy techniques such as IMRT (Intensity Modulated Radiotherapy) and IGRT (Image Guided Radiotherapy) made it possible to produce treatment plans and to conduct irradiation with precision of 1mm.In case of many benign tumors and majority of metastases, conventional imaging has precision adequate to requirements of radiotherapy.Special quality assurance (QA) of MRI and CT data together with QA related to image fusion techniques must be provided for IMRT and IGRT treatment.All gliomas infiltrate surrounding brain structures.Advanced MRI (Magnetic Resonance Imaging) techniques together with PET (Positron Emission Tomography) using specific tracers, have to be applied in order to visualize tumor/tumor borders.The most robust MRI techniques are PWI and DTI.3D Magnetic Resonance Spectroscopy is very promising tool that may be applied to RT planning.Regions of increased signals of lactic acid may be related to hypoxia, the most probable reason of gliomas radioresistance.Increased signals of choline may be related to regions increased proliferation.It is not clear, how to include metabolic information in treatment of glioma patients.The possibilities are: to increase radiation dose in regions of altered metabolic activity, the use of new drugs, antiangiogenic agents, hypoxia modifiers.Novel trial may address these questions but advanced imaging have to be used during these trials in order to join the treatment individualization of novel drug treatment with potential and precision of modem radiotherapy techniques.