The β2 adrenergic receptor (β2AR) constitutes the largest class of both human membrane proteins and drug targets, it relies on its ability to adopt multiple conformations, the conformational change from an inactive state to an active state (or in verse) enables the receptor to transmit a signal from the extracellular ligand-binding site to an intracellular G protein, thereby initiating diverse intracellular signaling processes.The study of the active and inactive states transition of B2AR can represent new functionally relevant models in the development of novel β2AR ligands.Thereby, we, in the work, use target molecular dynamics (MD) simulation to study the activation transition pathway of β2AR.