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This study aims to explore the protective effects and mechanisms of Lianshengjinhua (LSJH) on the renal fibrosis of mouse subjected to unilateral urethral obstruction (UUO).A total of 20 male C57BL/6 mouse were divided in random into two groups: UUO group (n=10), the UUO+LSJH group (n=10).Five mice from each group were sacrificed at time points (14 and 21 d) after operation and contralateral unobstructed kidney served as the control.