【摘 要】
：
AIM to explore the effects of solanine on Km and Vmax of NATase of HepG2.METHODS employing HPLC,using 2-AF as substrate,taking concentration of 2-AF as conc
【机 构】
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CenterfortheStudyofLifeSciences&EnvironmentalSciences,HarbinUniversityofCommerce,Harbin150076China
【出 处】
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International Conference on Molecular Machines and Sensors(2
论文部分内容阅读
AIM to explore the effects of solanine on Km and Vmax of NATase of HepG2.METHODS employing HPLC,using 2-AF as substrate,taking concentration of 2-AF as concentration of substrate,in intact HepG2 cells and their cytoplasm,taking the speed of 2-AF being acetylated to 2-AFF by NATase as the rate of NATase,using double reciprocal plot,taking 1/S(the reciprocal of concentration of 2-AF) and 1/V(reaction rate of NATase) as coordinates,got regression equation,calculated Km and Vmax.
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