Actin is a major component of the cytoskeleton of plant cells, and is involved in many important physiological processes.To understand the molecular mechanism of drought resistance in pea seedlings and select pea cultivars of drought tolerant, we analyzed the changes of PEAc3 expression of actin isoforms in pea seedlings under different concentrations of PEG6000 by using semi quantitative RT-PCR and real-time fluorescence quantitative RT-PCR technique.