Ion channels are involved in the migration of tumor cells that is required for their invasion and metastasis.In this present study, we describe the role of TRPM7 channel plays in the migration of A549 lung cancer cells.The TRPM7 currents in A549 cells were first characterized by using means of electrophysiology, pharmacology and RNA interference, and the expression of TRPM7 in A549 cells were also confirmed in mRNA and protein levels.TRPM7 currents in A549 cells have characteristics similar to that of TRPM7 currents reported, with strong outward rectification and sensitive to the inhibition of internal and external divalent cations.Epidermal growth factor (EGF), an important player in cancer development, greatly enhanced the migration ofA549 cells, and at the same time markedly up-regulated the expression of TRPM7 protein and the current amplitude of TRPM7.Depression of TRPM7 function with RNA interference or pharmacological agents no only reversed the EGF-enhanced migration ofA549 cells but also inhibited the basal migration ofA549 cells in the absence of added EGF.Furthermore, the EGF-induced reorganization of cytoskeleton ofA549 cells was also prevented by blocking of TRPM7 function.Thus it seems that TRPM7 plays a pivotal role in the migration ofA549 cells and TRPM7 could be a potential therapeutic target in lung cancers.