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Objective: To delineate the distribution, prognostic value and functional status ofB cells in human HCC.Methods: Immunohistochemistry was used to investigate the distribution and clinical significance of infiltrating CD20+ Bcells in a series of 120 HCC patients.The results were further tested in an independent series of 200 HCC patients.Fresh HCC specimens were used for isolation of tumor-infiltrating lymphocytes by flow cytometry sorting.The functional status of CD20+ B cells was determined by flow cytometry, ELISA,immunofluorescence and in vitro co-culture assay.