【摘 要】
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Objective:Compare Pueraria isoflavones and berberine total alkaloid use alone and proportion effecet on hypoglycemic,and serum chemical composition.Method:Ig given isoflavones (G) 1000mg·kg-1,total al
【机 构】
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Institute of National Medicine,Southwest University for Nationalities,Chengdu 610041,China
【出 处】
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世界中医药学会联合会中药药理专业委员会第七届学术会议、全国中药药理联合会第二届学术年会暨第20届中日健康学术研讨会
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Objective:Compare Pueraria isoflavones and berberine total alkaloid use alone and proportion effecet on hypoglycemic,and serum chemical composition.Method:Ig given isoflavones (G) 1000mg·kg-1,total alkaloid berberine (H) 200mg·kg-1,proportion isoflavones and alkaloid(GH proportion)(isoflavones:total alkaloids / 200mg·kg-1:160mg·kg-1),compare hypoglycemic effect of gavage group G,group H and group GH ratio;using HPLC gradient elution method to compare the ratio soflavones and total alkaloid in the chemical composition of the drug in serum.The results:Isoflavones and total alkaloid berberine alone and in combination can significantly reduce diabetes rats fasting blood glucose induced by alloxan,improve serum triglyceride (TG) and cholesterol (TC),GH ratio was superior to Group G and H group;GH serum has 12 ingredients from the prototype,including puerarin,daidzin,coptisine,jatrorrhizine,palmatine and berberine,22 ingredients derived metabolites,including 6 metabolites from the metabolism of isoflavones,five metabolites by berberine alkaloid metabolism,11 metabolites comes from the interaction of isoflavones and total alkaloid berberine arrowroot,while two components were not detected in the serum.Conclusion:isoflavones combined with total alkaloid berberine hypoglycemic effect better than the active ingredient alone,probably because the main active ingredient into the blood caused by the interaction of content changes,effective newbom metabolic intermediate result.
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