【摘 要】
:
Objective:Compare Pueraria isoflavones and berberine total alkaloid use alone and proportion effecet on hypoglycemic,and serum chemical composition.Method:Ig given isoflavones (G) 1000mg·kg-1,total al
【机 构】
:
Institute of National Medicine,Southwest University for Nationalities,Chengdu 610041,China
【出 处】
:
世界中医药学会联合会中药药理专业委员会第七届学术会议、全国中药药理联合会第二届学术年会暨第20届中日健康学术研讨会
论文部分内容阅读
Objective:Compare Pueraria isoflavones and berberine total alkaloid use alone and proportion effecet on hypoglycemic,and serum chemical composition.Method:Ig given isoflavones (G) 1000mg·kg-1,total alkaloid berberine (H) 200mg·kg-1,proportion isoflavones and alkaloid(GH proportion)(isoflavones:total alkaloids / 200mg·kg-1:160mg·kg-1),compare hypoglycemic effect of gavage group G,group H and group GH ratio;using HPLC gradient elution method to compare the ratio soflavones and total alkaloid in the chemical composition of the drug in serum.The results:Isoflavones and total alkaloid berberine alone and in combination can significantly reduce diabetes rats fasting blood glucose induced by alloxan,improve serum triglyceride (TG) and cholesterol (TC),GH ratio was superior to Group G and H group;GH serum has 12 ingredients from the prototype,including puerarin,daidzin,coptisine,jatrorrhizine,palmatine and berberine,22 ingredients derived metabolites,including 6 metabolites from the metabolism of isoflavones,five metabolites by berberine alkaloid metabolism,11 metabolites comes from the interaction of isoflavones and total alkaloid berberine arrowroot,while two components were not detected in the serum.Conclusion:isoflavones combined with total alkaloid berberine hypoglycemic effect better than the active ingredient alone,probably because the main active ingredient into the blood caused by the interaction of content changes,effective newbom metabolic intermediate result.
其他文献
目的:研究参附汤血中移行成分对血管紧张素Ⅱ(AngⅡ)所致心肌细胞凋亡及自噬的影响.方法:采用原代培养乳鼠心肌细胞,随机分为空白组、AngⅡ组、移行成分组.运用MTT比色实试验法测定心肌细胞存活率,采用实时定量PCR检测凋亡相关基因Caspase-3和自噬相关基因Beclin1、Atg5mRNA水平在不同组中的表达.结果:与正常组比较,血中移行成分组细胞存活率无明显变化(P>0.05),与模型组比
目的:探讨长期血糖平均水平不同的2型糖尿病(Type 2 diabetes mellitus,T2DM)患者下丘脑-垂体-肾上腺(Hypothalamic-pituitary-adrenal,HPA)轴激素与褪黑素(Melatonin,MLT)的关系,了解褪黑素在糖尿病发病进程中的变化.方法:将90例2型糖尿病患者根据糖化血红蛋白(HbA1c)水平分为三组(A组:HbA1c<7%,B组:7%≤Hb
目的:观察米非司酮(mifepristone,MIF)对慢性应激大鼠脂代谢和肝脏中GRmRNA、PPAR-γmRNA表达的影响.方法:采用高脂饲料联合四种不同的应激方式(悬尾30 min;PVC筒固定2h;纱布束缚2h;大鼠固定架制动2h)建立慢性应激大鼠模型.随机分为正常对照(control)组、应激模型(model)组、米非司酮-低剂量(MIF-L)(10 mg·kg-1·d-1)、中剂量(M
目的:观察米非司酮(mifepristone,MIF)不同剂量对高脂饲料喂养联合小剂量链脲佐菌素(streptozotocin,STZ)诱导的2型糖尿病大鼠血浆中激素水平(CRH、ACTH、CORT、INS、ALD)和海马组织中GRmRNA表达的影响,探讨米非司酮基于HPA轴功能调节治疗2型糖尿病的作用机制.方法:采用高脂饲料加小剂量链脲佐菌素(STZ,30mg·kg-1)腹腔注射诱导2型糖尿病模
目的:观察褪黑素(Melatonin,MLT)对慢性应激及高脂饲料喂养导致的胰岛素抵抗大鼠糖代谢和脂肪组织中GLUT-4、Leptin mRNA基因表达的影响,探讨其改善胰岛素抵抗的机制.方法:采用高脂饲料联合四种不同的应激方式,造成大鼠胰岛素抵抗.大鼠随机分为正常对照(Control)组、应激模型(Model)组、MLT-低剂量(MLT-L)(10 mg·kg-1·d-1)、MLT-中剂量(ML
目的:观察荔枝核有效部位群(SLEC,含总皂苷、黄酮和鞣质)改善2型糖尿病胰岛素抵抗的作用效果,并探讨其作用机制.方法:建立高脂饮食加链脲佐菌素诱导2型糖尿病大鼠动物模型,随机分为模型组、SLEC高剂量组(1.87g/kg)、荔枝核SLEC低剂量组(0.47g/kg)和盐酸罗格列酮组(3.87mg/kg),并设空白对照组,给药4周,观察SLEC对实验大鼠糖脂代谢、胰岛素抵抗和胰组织病理改变及超微结
目的:研究血管内皮生长因子(VEGF)在模拟不同海拔高原缺氧小鼠肺组织中的表达及意义.方法:采用低压低氧动物实验舱分别模拟海拔6000米和8000米高原缺氧环境,将BALB/c小鼠随机分为正常对照组,缺氧6h组、9h组、12h组、24h组、48h组、72h组,每组10只小鼠.HE染色切片观察缺氧不同时间小鼠肺组织的组织病理学改变情况,免疫组织化学法检测肺组织VEGF的表达水平差异及其规律.结果:缺
Atherosclerosis is a multifactofial and progressive disease commonly correlated with high fat diet.The aim of this study was to identify potential biomarkers for the early diagnosis and progression of
Objectives:Seek for the lipid biomarkers of lipidomics for HLP patients with "gan-yu-pi-xu" syndrome by study the lipidomics of HLP with "gan-yu-pi-xu" syndrome.To analysis the foundation of HLP with
Aim of study:To observe effect of Semen Litchi Effective Constituents (SLEC) on insulin resistance(IR) in rats with Type 2 Diabetes Mellitus(T2DM),and to explore its mechanism.Material and methods:T2D