Verticillium longisporum,a soil-borne pathogenic fungus causes vascular disease in oilseed rape(Brassica napus).We proposed that plant miRNAs are involved in the plant-Verticillium interaction.To identify oilseed rape miRNAs,we deep-sequenced two small RNA libraries made from V.ongisporum infected/non-infected roots and employed Brassica rapa and Brassica oleracea genomes as reference for miRNA prediction and characterization as well.