Development of an RNA In Situ Hybridization Assay for HCV Detection in Patient FFPE Samples

来源 :第十六届全军病理学术会议暨第十届全军病理专业委员会会议 | 被引量 : 0次 | 上传用户:qwerty_123asd
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Liver biopsy plays an important role for the management of patients with HCV, while HCV identification and genotype classification mainly depend on serum based tests,so we hypothesized that RNA in situ hybridization may present a valuable way to detect HCV infection in hepatocytes from liver biopsy.36 patient liver FFPE biopsy samples were obtained from302 Military Hospital of China in blinded fashion.Tissues were fixed in10% neutralbuffered formalin (NBF) for24 h and sectioned at a thickness of 5μm.
其他文献
Backgroud & Aims: Chronic hepatitis C is both a virological and progressive fibrotic disease.Recent studies demonstrated that IL-22 and IL-20, cytokine expressed by immune cells and hepatocytes, respe
会议
目的 分析和探讨前列腺间叶性肿瘤的临床病理特征.方法 对26例前列腺间叶性肿瘤的病例进行形态学观察、免疫组化染色及临床病理特征的分析.结果 2004-2016年解放军总医院病理科经前列腺穿刺活检及手术切除标本诊断前列腺肿瘤4691例,其中前列腺间叶性肿瘤26例(16例穿刺标本,10例手术标本),占总比例0.56%,原发性间叶性肿瘤24例(92%),包括前列腺间质肉瘤2例、孤立性纤维性肿瘤7例、横纹
目的 探讨去分化脊索瘤的临床病理特点、免疫表型及鉴别诊断。方法 结合文献,对4例去分化脊索瘤的临床资料、病理特征、免疫表型及治疗预后进行综合分析。结果 患者男性3例,女性1例,年龄16-63岁;其中3例为反复复发;1例位于颅底咽旁,1例位于斜坡-蝶窦,1例位于颈椎,1例位于骶骨;随访病人,2例分别于手术后8个月、12个月去世,2例失随访。去分化脊索瘤典型的组织学结构为典型的脊索瘤中均可出现高级别梭
会议
目的:非酒精性脂肪性肝病(NAFLD)尤其是其关键阶段非酒精性脂肪性肝炎(NASH)已成为全球最重要的公共健康问题之一.病理检查是NASH诊断的"金标准",但属有创诊断,不易被患者接受.无创诊断是NASH诊治的必然发展方向,但目前缺乏令人满意的无创诊断及预测NASH指标.本研究探究血清色素上皮细胞衍生因子(PEDF)与NAFLD进展的相关性,无创性预测诊断NASH.方法:选取确诊NAFLD病例13
Objective: Alagille syndrome (ALGS), a rare liver disease, is an autosomal dominant multisystem disorder.We report three cases of children patients with ALGS to understand the clinical characteristics
会议
目的 白介素-22(Interleukin-22, IL-22)是IL-10细胞因子家族成员之一,主要由活化的Th细胞(Th1,Th17,Th22细胞)及固有免疫细胞(NK细胞,ILC细胞)分泌,作用于上皮细胞、角化细胞和肝细胞等.IL-22在肝组织中可发挥保护或致病作用.有研究显示,IL-22在小鼠酒精性肝病(alcoholic liver disease,ALD)模型中发挥保护作用,然而其在人
Body: Background and objective: Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases and affects more than one-third of the population in the world.Innate immune cells are
会议
Competing Interests: The authors have declared that no competing interests exist.Purpose: The liver contains abundant copies of mitochondrion, which play important roles in physiology and pathogenesis
会议
军事病理学是运用病理学理论和方法研究平、战时部队人员损伤的原因、病变发生发展规律及其致伤机理的一门学科,应战争伤害而生,随战争武器的发展而不断拓展。从古代冷兵器战争到近代热兵器及机械化战争再到现代高技术信息化战争,不同的战争阶段军事病理学的研究技术手段和内容等各不相同,但均有其突出特征。冷兵器战争以刀、矛和弓箭为主要武器,刀伤、刺伤、切割伤为主要伤类,可造成单纯性骨折;热兵器、机械化、高技术和信息
会议
BACKGROUND: Nonalchoholic fatty liver disease (NAFLD) is a worldwide health problem of clinical significance and increasing prevalence.Steatosis and fibrosis are characteristic features of NAFLD durin