【摘 要】
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In order to overcome the problems and difficulties associated with bubbles formation in capillary electrochromatography (CEC),a binary solvents delivery system consisting of 2 micro-flow pumps is used
【机 构】
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School of Pharmacy,Shanghai Jiaotong University,Shanghai,200240
【出 处】
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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
论文部分内容阅读
In order to overcome the problems and difficulties associated with bubbles formation in capillary electrochromatography (CEC),a binary solvents delivery system consisting of 2 micro-flow pumps is used to provide a pressurized flow complementary to the electroosmotic flow (EOF).The end result is called pressurized capillary electrochromatography (pCEC).In such a system,quantitative sample injection can be realized through a rotary-type of nano-injector with a 10 nL sample loop.In addition,it is feasible to carry out a binary solvent gradient separation in pCEC,similar to that in HPLC,by programming the composition of mobile phase.An innovative pCEC instrument was developed suitable for performing three mode of experiments,namely,pCEC,capillary LC (cLC) and capillary electrophoresis (CE).The instrument was coupled with UV/Vis,MS and LIF detectors successfully and used in many investigations including applications on pharmaceutical,biological,environmental analyses,as well as pharmarcokinetics and metabolomics.
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