DSGXXS phosphodegron dictates regulated phosphorylation,ubiquitination.and proteosome-mediated degradation of-catenin,I B and an increasing number of other proteins involved in cell cycle control and signaling pathways.Database searches revealed the existence of over 100 proteins harboring the DSGXXS degron.Pilot analysis of 6 proteins revealed that TAZ (WWTRI) is constitutively degraded by the proteosome.Examination of TAZ expression in human cancer cell lines revealed that TAZ protein levels correlate with the invasiveness of breast cancer cell lines,enabling us to demonstrate that TAZ is important for migration.invasion and tumorigenesis of breast cancer cells.TAZ,like YAP,is sufficient to transform NIH3T3 and MCFIOA cells as assessed by anchorage-independent growth in soft agar.As TAZ is a downstream target inhibited by the Hippo tumor suppressor pathway (Hippo pathway),S89A mutation rendered TAZ refractory to Hippo inhibition and became more tumorigenic.Mechanistically,we showed that interaction of TAZ with TEAD transcriptional factors is important for its transforming property.