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As one of the main phyla within the Bacteria,the Actinobacteria,have been demonstrated that are unexpectedly diverse and play important roles in soil,freshwater and marine ecosystems [1-4].However,little is known about the actinobactenal diversity and distribution in hot springs.In this study.Actinobacterial diversity was investigated in three hot springs [Tengchong Frog Mouth hot spring (in Tengchong,China).