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It is well known that morphological characteristics of cells, in particular cell size and granularity, are changed during process of apoptosis.These alterations can be detected by flow cytometry as decrease of intensity of cell forward light scatter (FS) proportional to cell size, and increase of side light scatter (SS) proportional to cell granularity.The morphological distinctions between live and apoptotic cells are reflected on dot-plot in FS/SS coordinate obtained by flow cytometric analysis as two separate regions corresponding live and apoptotic cells.Change of cell position at this plot (cytogram) from the "live cell area" to the "apoptotic cell area" can be considered to a first approximation as a "trajectory of apoptosis".