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This paper investigated the laser acupuncture treatment to the mice with the optical fiber acupuncture needle intra body.The optical fiber acupuncture needle were fabricated using the optical fiber imbeding into the metal capillary tube with needle tip,so the optical fiber laser acupuncture needle was easy into body for the laser treatment.The treatment with laser to mice at "Zusanli" were carried in this paper,and the results showed that the laser optical fiber needle treatment to the "Zusanli" acupoints could improve the tolerance time of mice to hypoxia by anti-hypoxia experiments.