【摘 要】
:
Iron oxide magnetic nanoparticles (MNPs) are widely used as diagnostic and therapeutic agents for biomedical applications.Quantitatively analyzing biodistributi
【机 构】
:
KeyLaboratoryofProteinandPeptidePharmaceuticals,InstituteofBiophysics,ChineseAcademyofSciences,Beiji
【出 处】
:
The 9th Asian Biophysics Association Symposium (ABA2015)(第九届
论文部分内容阅读
Iron oxide magnetic nanoparticles (MNPs) are widely used as diagnostic and therapeutic agents for biomedical applications.Quantitatively analyzing biodistribution, pharmacokinetics and organ clearance of MNPs in mouse models is important for understanding their in vivo behavior.In this study, we developed a novel histochemical method for visualizing unlabeled MNPs in mouse tissues by employing their intrinsic peroxidasemimicking activity, regarding which we reported previously that MNPs could catalyze the oxidation of peroxidase substrates to produce a color reaction at the site of MNPs (Gao et al.Nat.Nanotechnol.2007, 2, 577-5831).Based on this MNPs-peroxidase approach, we determined the biodistribution and organ clearance of MNPs by visualizing and quantifying the localization of MNPs within the main organs.Compared to traditional Prussian blue assay, this novel MNPs-peroxidase approach has higher sensitivity.In conclusion, the developed MNPs-peroxidase approach based on intrinsic peroxidase activity of iron oxide nanoparticles was used effectively for quantitative detection of MNPs in mice by histochemical staining.Presumably, other nanoparticles having intrinsic peroxidase activity could also be considered.
其他文献
Human lysozyme and several of its familial mutants form amyloid fibrils and are responsible for systemic amyloidosis affecting liver and kidney.It is known that
Formation of new synapses, or synaptogenesis, is critical for neuronal circuit formation in the brain.Neurexin-neuroligin interaction has been reported to induc
An alignment of sequence of yeast iso-1-cytochrome-c (y-cyt-c) with that of the horse cyt-c suggests that the former has five extra N-terminal residues (www.uni
Mitochondrial flashes (mitoflashes) represent multifaceted signaling events of single mitochondrion, including transient burst of superoxide, depolarization of
Tandem duplications and fusions of single genes led to magnificent expansions of the divergence of protein structures and functions over evolutionary timescales
Chlamydomonas reinhardtii is a single celled alga, which undergoes apoptosis in response to UV-irradiation.UVI31+ in C.reinhardtii exhibits DNA and RNA endonucl
Background and Objective:Anillin and Mid1 play key roles in organizing and stabilizing the contractile ring during cytokinesis in animals and fungi, respectivel
Most drugs exert pharmacological effects through interaction with their target proteins.Therefore, drug target identification is a crucial step towards the unde
Poor understanding of the basic biology of Mycobacterium tuberculosis (MTB), the etiological agent of tuberculosis, hampers development of much-needed drugs, va
Alzheimers disease is accompanied by the accumulation of senile plaques composed of insoluble amyloid beta (Aβ) peptides and neurofibrillary tangles (NFTs).Abe