【摘 要】
：
Objective: To investigate the characteristics of breast cancer stem cells and their protein expression of RecQ family helicases.Methods: (1) Breast cancer stem cell were screened and cultured in serum
【机 构】
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Department of Immunology, Basic Medical School De
【出 处】
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The 1st NI-CMI Joint Conference(第一届CMI-NI免疫学国际研讨会)
论文部分内容阅读
Objective: To investigate the characteristics of breast cancer stem cells and their protein expression of RecQ family helicases.Methods: (1) Breast cancer stem cell were screened and cultured in serum-free medium.(2) CD44+CD24-/low were detected with flow cytometry for breast cancer stem cells.(3) The mRNA expression of drug resistant gene ABCG2 was detected with RT-PCR in breast cancer MDA-MB-435 stem cells and control cells.(4) The nude mice model of carcinoma was established to verify and compare the tumorigenicity of breast cancer stem cells with MDA-MB-435 cells.
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