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Aims: To evaluate the role of RUNX3 in the pathogenesis of ITP.Methods: 47 active ITP patients, 18 ITP with remission and 26 age and gender matched healthy control were included.Peripheral blood monon
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Aims: To evaluate the role of RUNX3 in the pathogenesis of ITP.Methods: 47 active ITP patients, 18 ITP with remission and 26 age and gender matched healthy control were included.Peripheral blood mononuclear cells (PBMCs) were isolated from ITP and control for isolation of RNA and plasma which were used to measure mRNA level of RUNX3 and T-box transcription factor (T-bet) by quantitative real-time PCR and interferon g (IFN-g) level by ELISA.Meanwhile, protein was extracted from PBMCs for western blot analysis of RUNX3 expression.Results:A significantly higher expression of RUNX3, T-bet and plasma level of IFN-g was observed in active ITP patients compared to control.No differences were observed between ITP with remission and control.Furthermore, a positive correlation of RUNX3 with T-bet was found in active ITP patients.Conclusion: Aberrant expression of RUNX3 was associated with the pathogenesis of ITP and therapeutically targeting it might be a novel approach in ITP treatment.
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