Enzyme-linked immunosorbent assay (ELISA) has become the gold standard for laboratorial and clinical analysis and improving its detection sensitivity is the central concern for meeting the demand of early disease diagnosis.Use of enzyme-loaded particles as labels, with multiple enzymes immobilized on the surface of a single particle, presents a promising solution to improve ELISA detection signal and thus enhance sensitivity.However, the current particle systems share common limitations of a relatively low enzyme loading capacity and a significant loss of enzyme activity during immobilization process.