MiRNAs as tumor suppressor have been identified in variety of cancer,but the role of miRNAs involve in ARS (acute radiation syndrome) in Tibet minipig are poorly understood.Here,in order to study the relationship between miRNAs pattern and ARS,microarray chip technology was used for screening of ARS related miRNAs in Tibet minipig small intestine.Not castrated male Tibetan minipig were chose as animal model (n =54),and exposed to the total body X-ray radiation at 0,2,5,8,11,14 Gy (n=9/ group),The total miRNA was extracted and hybridized to microarray chip.