Enzyme machinery for active DNA demethylation in Arabidopsis

来源 :2013全国植物生物学大会 | 被引量 : 0次 | 上传用户:zy198187
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  Active DNA demethylation is an important part of epigenetic regulation in plants and animals.In Arabidopsis, the ROS1/DME subfamily of DNA glycosylases function to promote DNA demethylation through a base excision-repair pathway.These specialized bifunctional DNA glycosylases remove the 5-methylcytosine base and then cleave the DNA backnone at the abasic site using either β3 elimination or β, δ elimination, resulting in a gap that is then filled with an unmethylated cytosine nucleotide by as yet unknown DNA polymerase and ligase enzymes.
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