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Objective The study aimed to investigate the effect of advanced oxidation protein products (AOPPs)on the proliferation and differentiation of rat osteoblast-like cells (ROBCs).Methods ROBCs were incubated with AOPPs, Cell proliferation and ALP activity was measured.Osteocalcin expression were examined using RT-PCR and Western blot.ROS was detected by flow cytometry and fluorescent microscope.NF-kB p65 subunit was detected by Western blot.