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目的:观察新加良附方对移植性人胃癌细胞(BGC-823)Survivin与Caspase-3蛋白表达的影响。方法:建立移植性人胃癌动物模型,并将动物模型随机分为模型对照、5-Fu组及新加良附方高、中、低剂量5组。新加良附大、中、小剂量组给药量分别为10g/kg、5g/kg和2.5g/kg;5-Fu组给药剂量为17mg/kg;模型组给予等量无菌生理盐水。连续给药、给水15天处死模型小鼠分离肿瘤,瘤块称重,石蜡包埋,并将肿瘤组织切片,免疫组化法检测Survivin与Caspase-3蛋白表达。结果:新加良附方高、中剂量组能上调肿瘤组织Caspase蛋白表达,与模型对照组比较,有统计学意义(P<0.05);新加良附方高、低剂量组均能下调Survivin蛋白表达,与模型对照组比较,有统计学意义(P<0.05)。结论:新加良附方能通过上调肿瘤组织Caspase蛋白表达,下调Survivin蛋白表达,从而诱导胃癌细胞发生凋亡。预示新加良附方在抗肿瘤方面具有进一步深入研究价值。
Objective: To observe the effect of Xinjialiang Fufang on the expression of Survivin and Caspase-3 protein in transplanted human gastric cancer cells (BGC-823). METHODS: A transplantable human gastric cancer animal model was established. Animal models were randomly divided into model control, 5-Fu group, and Xinjia Liangfu high, middle, and low dose groups. The dosages of Xinjialiang in the large, medium, and small dose groups were 10 g/kg, 5 g/kg, and 2.5 g/kg, respectively; the doses in the 5-Fu group were 17 mg/kg; the model group was given equal volume of sterile saline . The mice were sacrificed by continuous administration and water-feeding for 15 days. The tumor masses were weighed, embedded in paraffin, and the tumor tissues were sectioned. The expression of Survivin and Caspase-3 protein was detected by immunohistochemistry. Results: Xinjialiang Fufang high-and middle-dose group can up-regulate the expression of Caspase protein in tumor tissue, and it has statistical significance compared with the model control group (P<0.05). Xinjialiangfu high and low dose groups can down-regulate Survivin Protein expression was statistically significant compared with the model control group (P<0.05). Conclusion: Xinjia Liang Fu Fang can induce the apoptosis of gastric cancer cells by up-regulating the expression of Caspase protein in tumor tissue and down-regulating the expression of Survivin protein. It is predicted that Xinjialiang Affiliation has further research value in anti-tumor.