【摘 要】
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Objective Sendai virus(SeV)infection is strictly controlled in laboratory animals.The serological detection methods including ELISA and immunofluorescence Assay(IFA)are used in monitoring SeV infectio
【机 构】
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Institute of Laboratory Animal Science,Chinese Academy of Medical Science;Comparative Medicine Cente
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Objective Sendai virus(SeV)infection is strictly controlled in laboratory animals.The serological detection methods including ELISA and immunofluorescence Assay(IFA)are used in monitoring SeV infection.To some extent,the uncertainty of ELISA results and the subjective IFA judgment may affect the diagnoses of the SeV infection.In the current study,a serological immune-blot method was developed to analyze the SeV infection in rats.Methods The SeV antigens were harvested from SeV infected BHK-21 cells by supersonic lysis and were separated on an SDS-PAGE,and then transferred to an NC-membrane.1∶100 diluted rat serum samples were incubated with the membrane.Hrp-anti-Rat antibody was used to detect the specific bindings.Results Two specific bands were shown in positive samples but no in negatives.Some indeterminate samples by IFA and ELISA showed the weak SeV specific bands.Conclusion the western blot(WB)serological analysis shows its possible application in the monitoring of rat SeV infection.
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