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Alpha-MMC and MAP30 from Momordica charantia have been confirmed to possess anti-tumor and anti-virus activities.In order to solve the problems of requirement of a large amount of the two proteins for the subsequent research,the purpose of this study was to develop and optimize a single process with a combination of SP-sepharose FF, Superdex 75 and Macro-Cap-SP chromatogrphies suitable for laboratoryscale production of both Alpha-MMC and MAP30.