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A simple and effective large-scale preparation ofmagnolol and honokiol from bark of Magnolia officinalis Rehd et Wils,which based on extraction was established.The residue from bark extracted with cyclohexane was dispersed and suspended in ethanol-water (10∶5,v/V) system to remove compounds other than targets to give an enriched magnolol and honokiol fraction.This fraction was dissolved in cyclohexane,then extracted selectively with different aqueous ethanol systems to give honokiol and magnonol fractions respectively.These fractions crystallized to yield magnolol and honokiol.