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5. 黄芪注射液对脑损伤大鼠细胞凋亡的影响

黄芪注射液对脑损伤大鼠细胞凋亡的影响

来源 :2013中国医师协会中西医结合医师大会 | 被引量 : 0次 | 上传用户：siquan

【摘 要】

：

目的 观察黄芪注射液对脑损伤大鼠脑细胞凋亡的影响.探索黄芪注射液对缺血性脑细胞损伤的保护作用.方法 采用大脑中动脉局灶性栓塞(MCAO)模型,Tunel法检测凋亡细胞,用半定量反转录聚合酶链反应法(RTPCR)检测Bcl-xL和Bcl-2基因,观察黄芪注射液对大鼠缺血再灌注后脑细胞凋亡及Bcl-xL和Bcl-2蛋白的表达.结果：与模型组相比,黄芪注射液可显著降低脑细胞的凋亡数；增加Bcl-xL和B

【作 者】

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徐彬 章水晶 梁顺利 张宇燕 万海同 

【机 构】

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浙江省新华医院 浙江中医药大学附属第二医院 浙江中医药大学 310005

【出 处】

：

2013中国医师协会中西医结合医师大会

【发表日期】

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2013年12期

【关键词】

：

黄芪注射液 缺血性脑损伤 细胞凋亡 Bcl-xL Bcl-2蛋白 

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　　目的 观察黄芪注射液对脑损伤大鼠脑细胞凋亡的影响.探索黄芪注射液对缺血性脑细胞损伤的保护作用.方法 采用大脑中动脉局灶性栓塞(MCAO)模型,Tunel法检测凋亡细胞,用半定量反转录聚合酶链反应法(RTPCR)检测Bcl-xL和Bcl-2基因,观察黄芪注射液对大鼠缺血再灌注后脑细胞凋亡及Bcl-xL和Bcl-2蛋白的表达.结果：与模型组相比,黄芪注射液可显著降低脑细胞的凋亡数；增加Bcl-xL和Bcl-2蛋白的表达(p＜0.01；p＜0.05或p＜0.01).结论 黄芪注射液可以调节大鼠脑细胞的凋亡数、Bcl-xL和Bcl-2蛋白的表达,达到对缺血性脑损伤大鼠的保护作用.

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