Aim of study:To investigate the effects of serum containing Qinbai on expression of NLRP3 inflammasome in RAW264.7 cells infected with Mycoplasma pneumonia (MP).Material and method:RAW264.7 cells were randomly divided into normal group,Mp model group and serum containing Qinbai group.RAW264.7 cells and MP strain were cultured utilizing normal methods.Preparation of serum containing Qinbai,with a multiplicity of infection for 1:10 MP stimulation were pre-incubated over serum containing Qinbai on RAW264.7 cells;each groups cells were collected in 4h,8h,16h,24h increments.The changes of NLRP3,ASC and Caspase-1 mRNA were detected by the method of.FQ-PCR.Results:Compared with the normal group,the.expressions of NLRP3,ASC and Caspase-1 mRNA were significantly increased in the model group in 8h,16h,24h (P< 0.05 or P < 0.01).Compared with the model group,the expressions of NLRP3,ASC and Caspase-1 mRNA were significantly reduced in serum contained Qinbai group infected in 16h,24h (P< 0.05 0r P < 0.01).Conclusions:The expressions of NLRP3 inflammasome associated genes could be up-regulated in RAW264.7 cells infected with MP and serum containing Qinbai could have inhibited them from being up-regulated.