A Direct Estimate of the Human T Cell Receptor Diversity by Exhaustive T-cell repertoire sequencing

来源 :中国免疫学会第九届全国免疫学学术大会 | 被引量 : 0次 | 上传用户:dfddfasfafjdkjfkekjk
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
The adaptive immune system uses several strategies to generate a repertoire of T-and B-cell antigen receptors with sufficient diversity to recognize the universe of potential pathogens.Accurate estimation of the diversity of TCRβ CDR3 sequences in the entire αβ T-cell repertoire would be useful in many immune study areas.However, the exact quantitative data on the actual size of the T cell repertoire are lacking.
其他文献
Coxsackievirus B3 (CVB3) is a common etiology of myocarditis with an increased morbility and mortality in males.We previously reported that differential polarization of macrophages contributed to sexu
会议
Neuropilin-1 (Nrp1) has recently been implicated in the regulation of CD4 T-cell immune responses in experimental autoimmune encephalitis.However, whether Nrp1 plays a role in T cell mediated allograf
会议
microRNA (miRNA)可以核苷酸序列特异性方式调控目的基因的表达,在基因表达水平实现对细胞功能的精确调控,但是有关miRNA是否能够调控免疫细胞功能并发挥抗移植排斥作用的研究尚少.我们通过构建miR29 sponge小鼠(GS29),以C57来源miR-29 sponge小鼠为受体、以BalB/c小鼠为供体,进行小鼠同种心脏移植,结果发现miRNA-29缺陷可显著延长小鼠同种心脏移植物存
By inhibiting target gene expression, microRNAs (miRNAs) play major roles in various physiological and pathological processes.miR-146a, a miRNA induced upon lipopolysaccharide (LPS) stimulation and vi
会议
Background: Both acute liver injury and chronic liver fibrosis can lead to a potentially life-threatening condition, liver failure.Orthotopic liver transplantation is the only effective treatment for
Purpose: Domain-negative mutations in the signal transducer and activator of transcription 3 (STAT3) gene result in the classical multisystem form of autosomal dominant hyper IgE syndrome (AD-HIES), w
会议
Objective: Systemic Lupus Erythematosus (SLE) and pemphigus are two representatives of pathogenic autoantibodydriven autoimmune diseases systemically and organ-specifically, respectively.Given the cri
目的:类风湿性关节炎(RA)患者的滑膜腔是一个低氧环境.本研究将通过对骨性关节炎(OA)和RA患者的关节滑膜成纤维样细胞(FLS)进行正常氧和低氧培养后,观察FLS表达G6PI和HIF-1α的变化情况.方法:原代培养OA和RA患者的滑膜成纤维细胞,分别在正常氧和3%氧环境中培养24小时,Real-time PCR检测G6PI、HIF-1 αmRNA表达水平,Western blot检测G6PI、H
会议
The germinal center (GC) reaction supports affinity-based B-cell competition and generates high-affinity bone-marrow plasma cells (BMPC).ICOS and ICOSL have long been know as crucial for GC response,
会议
研究背景:过继转输调节性T细胞的治疗目前已被认为是充满希望的治疗炎症性肠病的崭新策略,但调节性T细胞内的信号分子事件仍缺乏研究.蛋白酪氨酸磷酸酶SHP-2在有丝分裂,代谢,转录调节,及细胞迁移中发挥重要功能,但SHP-2在调节性T细胞中的功能还不清楚.目的:考察SHP-2对调节性T细胞的调控作用.