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Lepidoptera is a large order of insects, which have major impacts on humans as agriculture pests.Midgut was considered to be an important target for insect control.in our previous study, the chit42 gene from Metarhizium anisopliae was cloned and integrated into the chromosome of Trichoderma koningii.The Trichoderma transformants displayed increased chitinase and insecticidal activities.Besides, overexpression of the M.anisopliae chit42 gene with a chitin-binding domain (chBD) in T.koningii increases the biocontrol activity, particularly the virulence against the Asian corn borer larvae.