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  5. SIRT1 enhances therapeutic efficacy of aged mesenchymal stem cells in rat myocardial infarction via

SIRT1 enhances therapeutic efficacy of aged mesenchymal stem cells in rat myocardial infarction via

来源 :第十二届中国介入心脏病学大会(CIT2014) | 被引量 : 0次 | 上传用户：bittermonkey
【摘 要】
：
Background Mesenchymal stem cells (MSCs) hold great promise for stem cell therapy and are gradually used in clinical trials in recent years.However,advanced age is a negative factor for autologous MSC
【作 者】
：
Chen Huiqiang Liu Xianbao Wang Jian 
【机 构】
：
Cardiovascular Key Lab of Zhejiang Province,Department of Cardiology,the Second Affiliated Hospital,
【出 处】
：
第十二届中国介入心脏病学大会(CIT2014)
【发表日期】
：
2014年3期
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Background Mesenchymal stem cells (MSCs) hold great promise for stem cell therapy and are gradually used in clinical trials in recent years.However,advanced age is a negative factor for autologous MSCs transplantation.Methods For in vitro study,we evaluated cell senescence using SA-B-gal method while for evalulated the stress response we conducted H2O2-induced hypoxia/ischemia model in MSCs.The conditioned medium was collected and was added to HUVEC to observe the alteration of tube-formation between vectot-aged MSCs and SIRTl-aged MSCs.For experiments in vivo,we first conducted ischemic female heart model induced by left anterior descending (LAD) ligation.At day 3 after male cell transplantation,the cell survival was evaluated by SRY detection.At day 28 after cell transplantation,heart function and fibrosis were analyzed by ECHO and Masson staining respectively.Morover,vascular density was evaluatied by CD31 and SMA staining.
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