Disorganized Muscle family member (Dim: 1) belongs to immunoglobulin superfamily,and is a structural protein localized to the region of the muscle cell membrane around and between the dense bodies.Strong immunogenicity to host s immune system was induced by Dim: 1 from Ascaris suum,which indicated that Dim: 1 could be a potential candidate for vaccine.The homologues of Dim: 1 were also detected in nematodes Brugia malayi and Trichostrongylus colubriformis.However,information on the complete coding sequence and protection potential of this molecule in H.contortus is lacking.In this study,full lengthof Dim: 1 cDNA was cloned using a rapid amplification of cDNA ends (RACE) strategy and the DNA vaccine encoding Dim: 1 was tested for protection against experimental H.contortus infections in goats: Fifteen lambs were allocated into three trial groups.The animals of Dim: 1 group was vaccinated with a recombinant Dim: 1 DNA vaccine twice on day 0 and 14,and challenged with 5000 infective H.contortus L3 (third larval stage) on day 28.An unvaccinated positive control group was challenged with L3 at the same time.An unvaccinated negative control group was not challenged with L3.The results indicated Dim: 1 DNA vaccines were transcribed at local injection sites and expressed in vivo post immunizations respectively.Following L3 challenge,the mean eggs per gram feces (EPG) and worm burdens of Dim: 1 group were reduced by 45.7% and 51.1%,respectively.Significantly high levels of serum IgG,serum IgA,mucosal IgA,CD4+T lymphocytes and B lymphocytes of Dim: 1 group were produced.Increased numbers of blood eosinophils and lymphocytes and declined haemoglobin level after L3 challenge were observed in the Dim: 1 group.These data suggest that recombinant H.contortus Dim: 1 DNA vaccine induced partial immune response and has protective potential against goat haemonchosis.