Objective: Global amphibian declines are a part of the current biodiversity crisis.Previous work have shown that the outbreak of the infectious diseases caused by pathogenic microorganisms is the major cause of amphibian declines, but studies have shown that resident microflora on the surface of the amphibian play a role of protection, but whether the resident microflora on amphibian skin defense pathogens, it is not clear.In this study, we choose the representative species of amphibians in the northeast, Rana dybowskii, through the study of microflora on the surface of the healthy individuals in the natural field and, the use of 16S rDNA technology to analyze the surface the Permanent flora species, to explore the relationship between microflora in the Rana dybowskii surface microbial flora and habitats.Method: Collect the healthy Rana dybowskii surface microorganisms and the water, soil microorganisms in the habitat, cultured and isolated microorganisms and detected R.dybowskii surface microorganisms with 16S rDNA, use community-level physiological profiles (CLPPs)method analyze the relationship between the microflora on Rana dybowskii surface and natural environment in habitats.Results: This study identified 9 kinds bacteria belong to 4 families from Rana dybowskii surface,including the Enterobacteriaceae, Bacillaceae, Pseudomonadaceae and Sphingobacteriaceae.CLPPs data use SPSS.A cluster analysis resulted in two main groupings: one for all the environment samples and one for all the skin swab samples from the amphibians.Conclusion: This result suggests that bacteria from the environment colonization on the skin of amphibians, surface microbes are part of the microflora from habitat;microorganisms on the amphibians surface impact by environmental microorganisms, also reflects the status of the environmental microbiology group.These results provide ideas for the conservation and protection of the diversity of amphibians.