【摘 要】
：
目的：建立有效的钩端螺旋体PCR检测方法,并对树鼩、长爪沙鼠和灰仓鼠等三种新型实验动物进行感染情况调查.
方法：针对NCBI公布的钩端螺旋体序列,设计并筛选特异性引物,优化
【机 构】
：
中国食品药品检定研究院实验动物资源研究所,北京100050
论文部分内容阅读
目的：建立有效的钩端螺旋体PCR检测方法,并对树鼩、长爪沙鼠和灰仓鼠等三种新型实验动物进行感染情况调查.
方法：针对NCBI公布的钩端螺旋体序列,设计并筛选特异性引物,优化PCR体系,进行特异性和敏感性测试;并运用优化PCR方法对树鼩、长爪沙鼠和灰仓鼠样品进行检测.
结果：成功建立钩端螺旋体PCR检测方法,序列测定验证了该方法的特异性.普通级树鼩钩端螺旋体的阳性率为8.33％,普通级长爪沙鼠钩端螺旋体为100％,清洁级长爪沙鼠和清洁级灰仓鼠钩端螺旋体的阳性率为0％.
结论：本研究建立了钩端螺旋体PCR检测方法,调查了树鼩、长爪沙鼠和灰仓鼠三种新型实验动物的感染情况,为这三种实验动物的研究和使用奠定基础.
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