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Assessing the toxicity level,safety of food chemical contaminants and potential new drug candidates are nowadays carried out in in vivo (e.g.using animals) and in vitro (e.g.on cell lines) models.But,up to-date,very few intestinal cell models exist to mimic the interactions in the intestinal tract of animals and man.Among the existing ones,human cancerogenic cell lines like Caco-2 are widely used.As cancerogenic,these cell lines are not appropriate model to perform studies in normal,non-cancerogenic intestinal environment.The uptake and potential toxicity and activity in the vertebrates,occurs in the gastro-intestinal tract (GIT).Yet,few studies have attempted to explore the influence of intestinal metabolism on the bioavailability of the individual components.There is therefore an increasing interest to access its role in the fate of other chemicals present in human food and reaching the GIT.It becomes clear that some of the substances entering mucosal cells are able to finely tune the expression and/or activity of transport proteins involved in the absorption process.These effects may also strongly affect the bioavailability of other substances that may be totally unrelated,being in turn responsible for potentially damaging interactions.