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Metabolic studies of Traditional Chinese Medicine (TCMs) have been applied effectively in the major fields of TCM research.However, there are still limitations for metabolic study methods so far using either in vivo or in vitro models in TCM research: the in vivo method is resource and labors costly, and many trace components cannot even be evaluated.On the other hand, the in vitro method is expensive due to the high standard of experimental conditions.Therefore, it is really important to establish an alternative metabolic study method for TCMs, which would combine advantages of these methods and overcome compound shortages to make it available for even trace compounds analysis.