【摘 要】
：
In PTM protein separation,we have developed a number of separation techniques by means of?nano-technology and chemical biology.We have used mesopore nano-materi
【机 构】
：
DepartmentofChemistryandInstitutesofBiomedicalSciences,FudanUniversity,Shanghai
【出 处】
：
全国生物医药色谱及相关技术学术交流会(2012)
论文部分内容阅读
In PTM protein separation,we have developed a number of separation techniques by means of?nano-technology and chemical biology.We have used mesopore nano-materials to enhance the PTM proteome,such as phosphorylation and glycosylation proteins.We also developed isotope labbelling technique,e.g.,O18 to identify selectively glycopeptides and related glycans,qualitatively and quantitatively.We have reported many effective process for on-site protelysis by use of trypsin digestion on the nano-materials/mesoporous-materials or on the nano-magematerials,with extremely fast digestion time in less than 1 min even in a few seconds.In this presentation,we will show some improtant progress in the PTM proteome separation and identification.
其他文献
基于毛细管电泳功能多样性的特点,拓展了其除分离分析之外的应用研究.(1)基于峰漂移法原理,采用淌度描述化合物的迁移行为,建立了一种测定化合物脂水分配系数(lg Po/w)的峰漂
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been listed as the most toxic organic pollutant.However,report on its poisoning case was rare.In this study,toxic
A fingerprinting approach was developed by UPLC-PDA-MS/MS for quality control of processed Aconiti Radix.This fingerprinting approach was based on the processin
为了较好地解决硅胶整体材料和有机高分子聚合物整体材料各自存在的缺点,近年来无机-有机杂化硅胶整体材料逐步引起人们的关注.总体而言,现有的杂化整体柱制备方法与硅胶整体
安捷伦液相色谱技术的发展策略,是在确保技术领先的前提下,重点关注液相色谱技术性能的提升,工作流程的自动化,应用范围的拓展,及用户投资保护,降低使用成本等诸多方面.UHPLC
免疫分析方法由于具有快速、简便、灵敏度高、选择性好等优势,已广泛应用于生命科学、药物分析、临床诊断及环境监测领域.在免疫分析过程中,如何将更多的信号分子标记于同一
混合模式色谱(mixed-modchromatography,MMC)是一种色谱固定相与溶质间具有一种以上作用力的色谱方法.与传统色谱相比,他具有高分离度,高选择性,高样品负载量,高速度等优点,
本文以四甲氧基硅烷和甲基三甲氧基硅烷作为混合前驱体,尿素为致孔剂,采用溶胶-凝胶法制备毛细管硅胶整体柱,再用巯基丙基三甲氧基硅烷对其进行功能化修饰,然后通过Au与-HS作