In present study, a M-phase phosphoprotein 6(Mpp6) gene was obtained from the ovary and neurosecretory organ in eyestalk cDNA library of black tiger shrimp (Penaeus monodon).By PCR testing,comparisons of nucleotides showed 95% homologies between the PCR product and the partial sequencing.The full length black tiger shrimp Mpp6 (PmMpp6) cDNA have obtained, homology analysis of the deduced amino acid sequence of the PmMpp6 with other known Mpp6 sequences by MatGAT software revealed that the PmMpp6 was high homology with other invertebrates.A conserved signature sequence of the Mpp6 family was found in the PmMpp6 deduced amino acid sequence.Analysis of the tissue expression pattern of the PmMpp6 showed that the PmMpp6 mRNA was expressed in all tissues tested, with highest levels in lymphoid tissue and muscle.Furthermore,the Mpp6 expression was found to be different in six stages of ovarian development.