Objective The immature and light-insensitive retina spontaneously generates a pattern of rhythmic bursting activity.Spatially,the activity is seen to spread across the retina in the form of waves and this kind of waves are named "Retinal wave".Retinal wave is a common phenomena which can be observed by calcium imaging in many kinds of neonatal animals retina.It has been proved that retinal waves are critical for refining retinal projections to their primary targets in the central visual system and involved in driving developmental processes within the retina itself.Direction-selective ganglion cell (DSGC) responses in different intensity to photo signals that moving in different directions.Its function has been developed quite well before bipolar cells send photo signals to the inner layer of retina,since the retinal waves progress in random different directions,the activity of retinal wave in the immature retina may play a role in the formation of direction-selective ganglion cells function.Our research is aimed to prove the relationship between retinal waves and the formation of direction-selective ganglion cells function in neonatal mouses retina.