【摘 要】
：
The tumorigenicity of human pluripotent stem cells (hPSCs) has been considered to be a major hurdle for their clinical applications.Here, we found that downregulation of miR-302 suppresses the tumorig
【机 构】
：
Center of Excellence in Tissue Engineering;Peking Union Medical College Chinese Academy of Medical S
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The tumorigenicity of human pluripotent stem cells (hPSCs) has been considered to be a major hurdle for their clinical applications.Here, we found that downregulation of miR-302 suppresses the tumorigenicity of hNT-2 cells (a human embryonal carcinoma pluripotent stem cell line).By gain-and loss-of-function approaches, we demonstrated that miR-302 promotes the proliferation and tumorigenicity of hPSCs through the dominant regulation of a set of cell cycle inhibitors and subsequent acceleration of the G 1 to S transition.Importantly, we also found that downregulation of miR-302 damages the self-renewal and pluripotency, and promotes differentiation of hPSCs.
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