Aim: Arteriosclerosis obliterans(ASO)of the lower extremities is a major reason of adult limb loss worldwide.Lacking of palpable symptoms results in the delay of therapy.Previous studies reveal that micorRNAs(miRNAs)participate in atherosclerosis,and it is easy to be detected.Therefore,the aim of current study is to screen a cluster of miRNAs that can be used as biomarker for ASO in the earlier stages.And we further study the function of such miRNAs in vitro.