Among all amphibian skin peptides,many isolated from different amphibian species have similarities in structure such that they can be classified into different families.However,the tryptophyllins (TPHs) are a special family in that they are small peptides with heterogeneous structures.Their name indicates a family of tryptophan-containing peptides,although not all contain this amino acid residue.Most of these TPHs have been found from the tree frog family Hylidae,predominantly South American leaf frogs from genus Phyllomedusa and Australian tree frogs from the genus Litoria.In recent years,more research focuses on discovery of TPHs from frog skin secretions and a few of them have been identified to have biological activities.This review gives a comprehensive and systematic introduction to the tryptophyllin peptides from frog skin for the first time,describes the TPHs discovery,the relations between the structures and functions and the acting receptor of some peptides from this family.