【摘 要】
：
目的：研究骨髓间充质干细胞(,MSCs)种植在型胶原支架材料,CG)上,软骨定向诱导后修复关节软骨缺损的可能性.方法：将来源于10只成年实验犬骨髓的贴壁细胞培养传代至第3代,收集后以2×106密度种植于直径9mm,厚3mm(干样品尺寸)干热交联处理(,DHT)的CG材料中,软骨诱导培养基诱导培养21d.观察每日细胞-材料复合体直径与初始直径的百分比,反应其收缩性.型胶原及平滑肌肌动蛋白(SMA)免
【机 构】
：
四川大学华西医院骨科 610041
【出 处】
：
2015成都国际骨科论坛暨四川省医学会第19次骨科学术会议
论文部分内容阅读
目的：研究骨髓间充质干细胞(,MSCs)种植在型胶原支架材料,CG)上,软骨定向诱导后修复关节软骨缺损的可能性.方法：将来源于10只成年实验犬骨髓的贴壁细胞培养传代至第3代,收集后以2×106密度种植于直径9mm,厚3mm(干样品尺寸)干热交联处理(,DHT)的CG材料中,软骨诱导培养基诱导培养21d.观察每日细胞-材料复合体直径与初始直径的百分比,反应其收缩性.型胶原及平滑肌肌动蛋白(SMA)免疫组织化学染色观测体外软骨形成情况.将体外复合体植入实验犬膝关节软骨缺损模型,12周观察.结果：诱导培养过程中细胞-材料复合体直径随时间延长而下降.21d后,细胞-材料复合体收缩至初始直径的64.4％±0.3％；组织学见：材料的孔隙收缩,新合成的基质使细胞-材料复合体的多数区域变为实体组织；型胶原及SMA染色阳性.植入实验犬膝关节软骨缺损12周后,犬膝关节功能恢复,关节软骨缺损处有软骨样组织填充.结论：将MSCs种植于CG材料中,经软骨诱导培养后并植入软骨缺损后能形成含有Ⅱ型胶原的软骨样实体组织.
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