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The increasing prevalence of obesity worldwide is associated with a parallel increase in non-alcoholic fatty liver disease (NAFLD).To investigate the effect of Lactobacillus johnsonii BS15 on NAFLD,120 male ICR mice were randomly divided into four groups and administrated with BS15 (2 × 107 cfu/ 0.2 mLor 2 × 108 cfu/0.2mL) or phosphate buffered saline (PBS) throughout a 17-week experimental period.The mice were fed with normal chow diet (NCD) five weeks before the experimental period.