【摘 要】
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Objective To investigate the role of RGC-32 in the proliferation and apoptosis of pancreatic cancer cell line BxPC-3.Methods Pancreatic cancer cell line BxPC-3 was routinely cultured.RGC-32 expression
【机 构】
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Department of Gastroenterology, The First Affiliated Hospital of Nanchang University;Department of G
【出 处】
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The 14th Congress of Gastroenterology China(第十四届全国消化系病学术会议)
论文部分内容阅读
Objective To investigate the role of RGC-32 in the proliferation and apoptosis of pancreatic cancer cell line BxPC-3.Methods Pancreatic cancer cell line BxPC-3 was routinely cultured.RGC-32 expression plasmid pcDNA3.1/myc-His C-RGC-32 was constructed and was transiently trasfected into BxPC-3 cells to overexpress RGC-32.Emptly vector (pcDNA3.1/myc-His C) was used as a negative control.Quantitative reverse transcription-PCR (qRT-PCR) and western blot were performed to determine the transfection efficiency.Cell counting kit-8 (CCK-8) and flow cytometry were employed to determine the influence of RGC-32 on cell proliferation and apoptosis of BxPC-3 cells respectively.
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