戊型肝炎病毒Ch-S-1株全长体外转录载体的构建及表达初步研究

来源 :第十一届全国病毒学学术研讨会暨第六届武汉现代病毒学国际研讨会 | 被引量 : 0次 | 上传用户：emeng

【摘要】

：

目的：构建出HEV Ch-S-1株的全长cDNA克隆,并转染至HepG2细胞并对其表达进行初步研究。方法：利用重叠PCR方法获得HEV Ch-S-1株全长PCR产物；构建体外转录载体pKS-HEV,成功获得HEV基因组RNA,并转染至HepG2细胞,通过RT-nPCR、套式PCR和间接免疫荧光实验检测病毒的表达。

【作者】

：

陈爽朱光泽李一权兰添范园园张诺娜胡宁宁邢彬李霄金宁一

【机构】

：

军事医学科学院军事兽医研究所,长春,130122 军事医学科学院军事兽医研究所,长春,130122

【出处】

：

第十一届全国病毒学学术研讨会暨第六届武汉现代病毒学国际研讨会

【发表日期】

：

2015年6期

【关键词】

：

戊型肝炎病毒反向遗传学全长cDNA克隆表达载体

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戊型肝炎病毒Ch-S-1株全长体外转录载体的构建及表达初步研究

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